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Ki67 antibody

CAT: 0800-F54499-0.05MLSize: 0.05 mLDry Ice: NoHazardous: No
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CAT#:0800-F54499-0.05MLSize:0.05 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1:25
UniProt
P46013
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the Ki67 antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
2179CT8.1.2.1
Applications
IHC-P
Purity
Protein G affinity
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the Ki67 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Ki67 antibody is available for research use only.
Storage Conditions
Aliquot the Ki67 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the Ki67 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear
Image Legend
IHC testing of FFPE human tonsil tissue with Ki67 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.

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