Histone H2AX Antibody / H2AFX

• Description:

  Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

• UniProt:

  P16104

• Host:

  Mouse

• Immunogen:

  A portion of amino acids 115-143 from the human protein was used as the immunogen for the Histone H2AX antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Applications:

  IHC-P, WB

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the Histone H2AX antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Histone H2AX antibody is available for research use only.

• CAS Number:

  9007-83-4