PIGR Antibody

• Description:

  PIGR binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.

• Specifications:

  Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

• UniProt:

  P01833

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 646-672 from the human protein was used as the immunogen for the PIGR antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  FACS, IF, IHC-P, WB

• Purity:

  Antigen affinity purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the PIGR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This PIGR antibody is available for research use only.

• Storage Conditions:

  Aliquot the PIGR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the PIGR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  IHC testing of FFPE human colon tissue with PIGR antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.