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Phospho-CREB Antibody (pS133)

CAT: 0800-F54407-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
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CAT#:0800-F54407-0.08MLSize:0.08 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CREB is a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, (CRE), a sequence present in many viral and cellular promoters. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000
UniProt
P16220
Host
Rabbit
Reactivity
Human
Immunogen
The amino acids surrounding phosphorylated serine 133 from the human protein were used as the immunogen for the phospho-CREB antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC-P, WB
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the phospho-CREB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This phospho-CREB antibody is available for research use only.
Storage Conditions
Aliquot the phospho-CREB antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the phospho-CREB antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear
Image Legend
Western blot testing of lysate from HeLa cells treated or untreated with TPA, using phospho-CREB antibody.
Product Datasheet
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