CREB Antibody

• Description:

  CAMP responsive element binding protein 1, is a protein that in humans is encoded by the CREB1 gene. This protein binds the cAMP response element, a DNA nucleotide sequence present in many viral and cellular promoters. The binding of CREB1 stimulates transcription. This transcription factor is a member of the leucine zipper family of DNA-binding proteins. It binds as a homodimer to the cAMP-responsive element, an octameric palindrome. CREB is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway.

• Specifications:

  Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cels

• UniProt:

  P16220

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Human partial recombinant protein (AA 1-177) was used as the immunogen for this CREB antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, FACS

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the CREB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This CREB antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the CREB antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the CREB antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Flow cytometry testing of fixed and permeabilized human 293T cells with CREB antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= CREB antibody.