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CREB Antibody

CAT: 800-R31525Size: 100 µgDry Ice: NoHazardous: No
100 µgSelected
PRICE
AVAILABILITY
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CAMP responsive element binding protein 1, is a protein that in humans is encoded by the CREB1 gene. This protein binds the cAMP response element, a DNA nucleotide sequence present in many viral and cellular promoters. The binding of CREB1 stimulates transcription. This transcription factor is a member of the leucine zipper family of DNA-binding proteins. It binds as a homodimer to the cAMP-responsive element, an octameric palindrome. CREB is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cels
UniProt
P16220
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Human partial recombinant protein (AA 1-177) was used as the immunogen for this CREB antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the CREB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This CREB antibody is available for research use only.
Storage Conditions
After reconstitution, the CREB antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting points. Titration of the CREB antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Flow cytometry testing of fixed and permeabilized human 293T cells with CREB antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= CREB antibody.

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