Bcl-W Antibody (BH3 domain specific)

• Description:

  Bcl-w is a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis.

• Specifications:

  Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25

• UniProt:

  Q92843

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  A portion of amino acids 24-59 from the human protein was used as the immunogen for the Bcl-W antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, IHC-P

• Purity:

  SAS precipitation

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the Bcl-W antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Bcl-W antibody is available for research use only.

• Storage Conditions:

  Aliquot the Bcl-W antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the Bcl-W antibody (BH3 Domain Specific) may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  IHC testing of FFPE human breast cancer tissue with Bcl-W antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.