Sclerostin Antibody / SOST

• Description:

  Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.

• Specifications:

  Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

• UniProt:

  Q9BQB4

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A portion of amino acids 12-42 from the human protein was used as the immunogen for the Sclerostin antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  IHC-P, WB

• Purity:

  Antigen affinity purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the Sclerostin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Sclerostin antibody is available for research use only.

• Storage Conditions:

  Aliquot the Sclerostin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the Sclerostin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of human 1) kidney and 2) liver lysate with Sclerostin antibody. Predicted molecular weight ~24 kDa.