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PKM2 Antibody

CAT: 0800-F54017-0.1MLSize: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0800-F54017-0.1MLSize:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. [UniProt]
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, ICC/IF: 1:400
UniProt
P14618
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
A human recombinant partial protein was used as the immunogen for this PKM2 antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
1A7-G6-H6
Applications
WB, ICC/IF
Purity
Protein G affinity
Format
Purified
Buffer
Antibody in PBS with 50% Glycerol and 0.03% ProClin 300
Reconstitution
Store the PKM2 antibody at -20oC.
Limitations
This PKM2 antibody is available for research use only.
Storage Conditions
Store the PKM2 antibody at -20°C.
Formulation
In PBS with 50% glycerol and 0.03% ProClin 300
Applications Notes
The stated application concentrations are suggested starting points. Titration of the PKM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of monkey COS7, rat PC12, rat C6, mouse NIH3T3 and human HeLa cell lysates with PKM2 antibody at 1:1000. Predicted molecular weight ~58 KDa.

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