GARS Antibody

• Description:

  Glycine--tRNA ligase catalyzes the attachment of glycine to tRNA (Gly) . Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs.

• Specifications:

  Western blot: 1:1000-2000, FACS (intracellular) : 1:25

• UniProt:

  P41250

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A human recombinant protein of amino acids 15-305 was used as the immunogen for the GARS antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  1641CT837.23.96

• Applications:

  WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the GARS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This GARS antibody is available for research use only.

• Storage Conditions:

  Aliquot the GARS antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the GARS antibody may be required due to differences in protocols and substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of human 1) Jurkat, 2) Ramos and 3) HT-1080 cell lysate with GARS antibody at 1:2000. Predicted molecular weight: 83 kDa.