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Zebrafish Gfap Antibody

CAT: 0800-F52537-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
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CAT#:0800-F52537-0.08MLSize:0.08 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Zebrafish GFAP shares significant structural and functional homology with mammalian GFAP, including a conserved α-helical rod domain crucial for filament assembly. It is prominently expressed during embryonic development and persists in specific glial populations in the adult brain and spinal cord. Unlike in mammals, radial glia expressing GFAP remain active as neural stem cells in the adult zebrafish CNS, making GFAP an important marker for studying neurodevelopment and regeneration. GFAP expression in zebrafish is widely used as a glial cell marker in developmental biology, neural stem cell research, and models of CNS injury and repair. Its upregulation is also indicative of reactive gliosis following CNS trauma.
CAS Number
9007-83-4
Specifications
IHC (Paraffin) : 1:25, Western blot: 1:1000
UniProt
Q58EE9
Host
Rabbit
Reactivity
Zebrafish
Immunogen
This Gfap antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 2-36 amino acids from the N-terminal region of zebrafish Gfap.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the Gfap antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Zebrafish Gfap antibody is available for research use only.
Storage Conditions
Aliquot the Gfap antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the Gfap antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
IHC analysis of FFPE zebrafish muscle section using Gfap antibody; Ab was diluted at 1:25.

UniProtKB · Q58EE9

Glial fibrillary acidic protein

GFAP_DANRE · Danio rerio

View on UniProt ↗
Primary accession
Q58EE9
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
gfap
Protein existence
1: Evidence at protein level
Organism
Danio rerio (Zebrafish)
Taxonomy ID
7955
Alternative names
GFAP
EC number
—
Processing
—
Secondary accessions
A2VCX6, Q5YLV6, Q6NUV9, Q6TH89, Q7T2N9, Q9PW34
Protein keywords

Domain

Coiled coil

Cellular component

CytoplasmCytoskeletonIntermediate filament

Technical term

Reference proteome

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