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GFAP Antibody

CAT: 0800-V9023SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V9023SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP) . It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P14136
Host
Mouse
Reactivity
Human
Immunogen
GFAP isolated from pig spinal cord was used as the immunogen for this GFAP antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPM507
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the GFAP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This GFAP antibody is available for research use only.
Storage Conditions
Store the GFAP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Location
Cytoplasmic
Image Legend
IHC: Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP antibody (SPM507) .

UniProtKB · P14136

Glial fibrillary acidic protein

GFAP_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P14136
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
GFAP
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
GFAP
EC number
—
Processing
—
Secondary accessions
A7REI1, B2RD44, D3DX59, E9PAX3, Q53H98, Q5D055, Q6ZQS3, Q7Z5J6, Q7Z5J7, Q96KS4, Q96P18, Q9UFD0
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

PTM

CitrullinationMethylationPhosphoprotein

Domain

Coiled coil

Cellular component

CytoplasmIntermediate filament

Disease

Disease variantLeukodystrophy

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