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SUMO-2 Antibody

CAT: 0800-F52344-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
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CAT#:0800-F52344-0.08MLSize:0.08 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins.
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1:25, Western blot: 1:1000
UniProt
P61956
Host
Mouse
Reactivity
Human, Rat
Immunogen
Purified His-tagged protein was used to produced this monoclonal SUMO-2 antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
973CT8.1.1
Applications
IF, WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the SUMO-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This SUMO-2 antibody is available for research use only.
Storage Conditions
Aliquot the SUMO-2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the SUMO-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Bovine, Chicken, Hamster, Mouse, Pig
Image Legend
Fluorescent image of HeLa cells stained with SUMO-2 antibody at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green) . Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red) .

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