Src Antibody

• Description:

  This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-Src kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

• Specifications:

  Western blot: 1:2000, IHC (Paraffin) : 1:50, Flow Cytometry: 1:50, Immunofluorescence: 1:50

• UniProt:

  P12931

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 24-52 from the human protein was used as the immunogen for this Src antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, IHC, FACS, IF, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the Src antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Src antibody is available for research use only.

• Storage Conditions:

  Aliquot the Src antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the Src antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Mouse

• CAS Number:

  9007-83-4

• Image Legend:

  Src antibody western blot analysis in (1) MCF-7 and (2) A431 lysate