ChAT Antibody

• Description:

  ChAT (Choline acetyltransferase) is an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease.

• Specifications:

  Western blot: 1:1000, Immunofluorescence: 1:10-1:50

• UniProt:

  P28329

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  A portion of amino acids 98-128 from the human protein was used as the immunogen for this ChAT antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  IF, WB, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the CHAT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This ChAT antibody is available for research use only.

• Storage Conditions:

  Aliquot the ChAT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the ChAT antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of ChAT antibody and NCI-H460 lysate. Predicted molecular weight ~83/74 kDa (isoforms M/S) .