UHRF1 Antibody

• Description:

  UHRF1 is a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone acetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint.

• Specifications:

  Western blot: 1:1000, Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50

• UniProt:

  Q96T88

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 229-257 from the human protein was used as the immunogen for this UHRF1 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  FACS, IF, WB, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the UHRF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This UHRF1 antibody is available for research use only.

• Storage Conditions:

  Aliquot the UHRF1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the UHRF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  UHRF1 antibody western blot analysis of HeLa lysate. Predicted/observed molecular weight: ~91/91-106kDa.