LATS1 Antibody

• Description :

  The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.

• Specifications :

  Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

• UniProt :

  O95835

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  A portion of amino acids 1-30 from the human protein was used as the immunogen for this LATS1 antibody.

• Clonality :

  Polyclonal

• Isotype :

  Ig

• Applications :

  WB, IHC, ELISA

• Purity :

  Purified

• Format :

  Purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the LATS1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This LATS1 antibody is available for research use only.

• Storage Conditions :

  Aliquot the LATS1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  Titration of the LATS1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Image Legend :

  Western blot analysis of LATS1 antibody and K562 lysate.