AKT2 Antibody

• Description:

  AKT2 is a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. AKT2 has been shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.

• Specifications:

  Immunofluorescence: 1:10-1:50, Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50

• UniProt:

  P31751

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 416-444 from the human protein was used as the immunogen for this AKT2 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  IHC, FACS, IF, WB, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the AKT2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This AKT2 antibody is available for research use only.

• Storage Conditions:

  Aliquot the AKT2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the AKT2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Mouse, Rat

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of AKT2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the human gene (2) . Predicted molecular weight: ~56kDa.