Rab5 Antibody

• Description:

  Rab5 is required for the fusion of plasma membranes and early endosomes.

• Specifications:

  Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50

• UniProt:

  P20339

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 182-211 from human Rab5a was used as the immunogen for this Rab5 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, IHC, IF, FACS, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the Rab5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Rab5 antibody is available for research use only.

• Storage Conditions:

  Aliquot the Rab5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the Rab5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  Rab5 antibody flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control (left histogram) . FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.