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Neuron Specific Enolase Antibody

CAT: 0800-F48200-0.4MLSize: 0.4 mLDry Ice: NoHazardous: No
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CAT#:0800-F48200-0.4MLSize:0.4 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NSE is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha to gamma enolase occurs in neural tissue during development in rats and primates. [Wiki]
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100
UniProt
P09104
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 213-239 from human ENO2 was used as the immunogen for this Neuron Specific Enolase antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Store at 4oC for up to one month. For long term, aliquot the Neuron Specific Enolase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Neuron Specific Enolase antibody is available for research use only.
Storage Conditions
Store at 4°C for up to one month. For long term, aliquot the Neuron Specific Enolase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the Neuron Specific Enolase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse, Rat
Image Legend
Western blot analysis of Neuron Specific Enolase antibody and Y79 lysate. Predicted molecular weight: ~47 kDa.

UniProtKB · P09104

Gamma-enolase

ENOG_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P09104
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ENO2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
4.2.1.11
Processing
—
Secondary accessions
B7Z2X9, Q96J33
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationHydroxylationIsopeptide bondPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneCytoplasmMembrane

Biological process

Glycolysis

Molecular function

Lyase

Ligand

MagnesiumMetal-binding

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