HSF1 Antibody

• Description :

  Heat shock transcription factor 1 (HSF1) mediates the induction of heat shock protein gene expression in cells exposed to elevated temperature and other stress conditions. In response to stress, HSF1 acquires DNA-binding ability and localizes to nuclear stress granules. SUMO modification of HSF1 converts HSF1 to the DNA-binding form. HSF1 colocalizes with SUMO-1 in nuclear stress granules, which is prevented by mutation of the HSF1 lysine targeted for sumoylation.

• Specifications :

  Immunofluorescence: 1:10-1:50

• UniProt :

  Q00613

• Host :

  Rabbit

• Reactivity :

  Human, Mouse

• Immunogen :

  A portion of amino acids 278-309 from the human protein (sumoylation site) was used as the immunogen for this HSF1 antibody.

• Clonality :

  Polyclonal

• Isotype :

  Ig

• Applications :

  IF, ELISA

• Purity :

  Purified

• Format :

  Purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the HSF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This HSF1 antibody is available for research use only.

• Storage Conditions :

  Aliquot the HSF1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  Titration of the HSF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity :

  Bovine

• CAS Number :

  9007-83-4

• Image Legend :

  Confocal immunofluorescent analysis of HSF1 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . DAPI was used as a nuclear counterstain (blue) .