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HSF1 Antibody

CAT: 0800-F48065-0.4MLSize: 0.4 mLDry Ice: NoHazardous: No
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CAT#:0800-F48065-0.4MLSize:0.4 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Heat shock transcription factor 1 (HSF1) mediates the induction of heat shock protein gene expression in cells exposed to elevated temperature and other stress conditions. In response to stress, HSF1 acquires DNA-binding ability and localizes to nuclear stress granules. SUMO modification of HSF1 converts HSF1 to the DNA-binding form. HSF1 colocalizes with SUMO-1 in nuclear stress granules, which is prevented by mutation of the HSF1 lysine targeted for sumoylation.
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1:10-1:50
UniProt
Q00613
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 278-309 from the human protein (sumoylation site) was used as the immunogen for this HSF1 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IF, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the HSF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This HSF1 antibody is available for research use only.
Storage Conditions
Aliquot the HSF1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the HSF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Bovine
Image Legend
Confocal immunofluorescent analysis of HSF1 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . DAPI was used as a nuclear counterstain (blue) .
Product Datasheet
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