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CD73 Antibody

CAT: 0800-F47794-0.4MLSize: 0.4 mLDry Ice: NoHazardous: No
CAT#:0800-F47794-0.4MLSize:0.4 mL
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Ecto-5-prime-nucleotidase/5-prime-ribonucleotide phosphohydrolase/NT5E/CD73 catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70 kD subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5E/CD73 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. It is not known whether the different enzymes are coded by different genes or result from different posttranslational modifications of a single coding sequence.
CAS Number
9007-83-4
Specifications
Western blot: 1:1000-2000, IHC (Paraffin) : 1:10-1:50
UniProt
P21589
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 520-550 from the human protein was used as the immunogen for this CD73 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the CD73 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CD73 antibody is available for research use only.
Storage Conditions
Aliquot the CD73 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the CD73 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of lysate from (1) U266, (2) HepG2 cell line, (3) mouse brain tissue lysate using CD73 antibody at 1:2000. Predicted molecular weight: 65-70 kDa.

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