Beclin 1 Antibody

• Description:

  Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole) . Beclin 1 plays a role in two fundamentally important cell biological pathways: autophagy and apoptosis. Beclin 1 is thought to function as a VPS and autophagy protein as part of a complex with Class III PI3 kinase, Vps34.

• Specifications:

  Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

• UniProt:

  Q14457

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  A portion of amino acids 181-210 from the human protein was used as the immunogen for this Beclin 1 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, IHC, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS pH 7.4 with 0.09% sodium azide

• Reconstitution:

  Aliquot the Beclin 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Beclin 1 antibody is available for research use only.

• Storage Conditions:

  Aliquot the Beclin 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the Beclin 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Bovine, Chicken

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of Beclin 1 antibody and 293 lysate transiently transfected with the BECN1 gene (2ug/lane) .