Beclin 1 Antibody

Bellini-1 is a protein that in humans is encoded by the BECN1 gene, also known as BECN1, ATG6, VPS30, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, autophagy-related gene 6 (ATG6) . Beclin 1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagosome in autophagy. The gene encodes a novel 450-amino acid protein containing a coiled-coil region, within which it has limited homology to myosin-like proteins. Northern blot analysis of mouse and adult human tissues revealed widespread expression. A 2.2-kb transcript was present at highest levels in human skeletal muscle and at detectable levels in all tissues examined. In some tissues, additional 1.7- and 1.4-kb transcripts were observed, suggesting the presence of alternatively spliced transcripts. Immunoperoxidase staining of human hippocampus and frontal cortex sections revealed Beclin 1 immunoreactivity in many neurons throughout these regions, as well as in some glial cells. It protects against infection by a neurovirulent strain of Sindbis virus that is known to overcome the protective effects of Bcl2. Liang et al. showed that Beclin 1 reduced Sindbis virus replication in mouse brain and reduced Sindbis virus-induced cell death in mouse brain.

9007-83-4

Western blot: 0.5-1 µg/mL

Q14457

Human, Mouse, Rat

Amino acids 222-237 (LDQEEAQYQREYSEFK) were used as the immunogen for this Beclin 1 antibody (100% homologous in human, mouse and rat) .

WB

Antigen affinity

Antigen affinity purified

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Store the lyophilized Beclin 1 antibody at 4oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.

This Beclin 1 antibody is available for research use only.

Store the lyophilized Beclin 1 antibody at 4°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Titration of the Beclin 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.