LIN28B Antibody

LIN28B Antibody

• Description: LIN28B is a suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA. Does not act on pri-miR21. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation. This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent. Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity). [UniProt]
• CAS Number: 9007-83-4
• UniProt: Q6ZN17
• Host: Rabbit
• Immunogen: A portion of amino acids 95-128 from the human protein was used as the immunogen for this LIN28B antibody.
• Clonality: Polyclonal
• Isotype: Ig
• Applications: WB, IHC, IF, FACS, ELISA
• Format: Antigen affinity purified
• Buffer: In 1X PBS, pH 7.4, with 0.09% sodium azide
• Reconstitution: Aliquot the LIN28B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
• Limitations: This LIN28B antibody is available for research use only.