Nucleolin Antibody

• Description:

  Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [Wiki]

• Specifications:

  Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Immunofluorescence: 1:10-1:50

• UniProt:

  P19338

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  The amino acid sequence used as immunogen for this Nucleolin antibody is found between 425-455 of the human protein.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, IHC, IF, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  The Nucleolin antibody should be aliquoted and and stored frozen at -20oC. Avoid repeat freeze-thaws.

• Limitations:

  This Nucleolin antibody is available for research use only.

• Storage Conditions:

  The Nucleolin antibody should be aliquoted and and stored frozen at -20°C. Avoid repeat freeze-thaws.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Due to differences in protocols and secondary/substrate sensitivity, it may be necessary to titrate the Nucleolin antibody for optimal performance.

• Prediction Reactivity:

  Primate

• CAS Number:

  9007-83-4

• Image Legend:

  Nucleolin antibody western blot analysis in HepG2 lysate. Predicted molecular weight is 77 kDa, observed size is 100~110 kDa.