KLF4 Antibody

• Description :

  KLF4 is a transcription factor that can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. [UniProt]

• Specifications :

  Western blot: 1:2000, Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50

• UniProt :

  O43474

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Recombinant protein was used to produce this monoclonal KLF4 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1

• Clone :

  56CT5.1.6

• Applications :

  WB, IF, FACS, ELISA

• Purity :

  Purified

• Format :

  Purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the KLF4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This KLF4 antibody is available for research use only.

• Storage Conditions :

  Aliquot the KLF4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  Titration of the KLF4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Image Legend :

  Immunofluorescence analysis with KLF4 antibody. HeLa cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at approximately 62 hours after transfection.