Nucleolin Antibody

• Description :

  Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. [UniProt]

• Specifications :

  Western blot: 1:500-1:1000, IHC (Paraffin) : 1:50-1:100, Immunofluorescence: 1:10-1:50

• UniProt :

  P19338

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  This Nucleolin antibody was produced from mice immunized with a protein from human NCL.

• Clonality :

  Monoclonal

• Isotype :

  IgG1

• Clone :

  130CT43.8.5

• Applications :

  WB, IHC, IF, ELISA

• Purity :

  Purified

• Format :

  Purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the Nucleolin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This Nucleolin antibody is available for research use only.

• Storage Conditions :

  Aliquot the Nucleolin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  Titration of the Nucleolin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Image Legend :

  Western blot analysis of Nucleolin antibody and 293 lysate. Predicted molecular weight is 77 kDa, observed size is 100~110 kDa.