ILK2/ILK1 Antibody

• Description:

  Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK) is an ankyrin repeat containing 51 kDa receptor-proximate serine-threonine kinase (1), with a reported migration rate of 59K. This 451 amino acid protein interacts with the cytoplasmic domain of the beta-1 integrin subunit and contains sequence motifs found in pleckstrin homology domains capable of interacting with phosphoinositide lipids. ILK is an upstream regulator of Pi (3) K dependant activation of protein kinase B (PKB/AKT) and inhibition of glycogen synthase kinase 3 (GSK-3) . ILK2 expression is associated with mediation of cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. ILK2 is overexpressed in some highly invasive tumor cell lines.

• Specifications:

  Western blot: 1:1000

• UniProt:

  Q13418

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  A portion of amino acids 391-421 from the human protein was used as the immunogen for this ILK2/ILK1 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS pH 7.4 with 0.09% sodium azide

• Reconstitution:

  Aliquot the ILK2/ILK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This ILK2/ILK1 antibody is available for research use only.

• Storage Conditions:

  Aliquot the ILK2/ILK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the ILK2/ILK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Rat, Bovine

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of ILK2/ILK1 antibody and HeLa lysate.