Products for Research Use Only

IL2RA/CD25 Antibody

CAT: 0013-GTR18225893Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18225893Size:500 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
IL2RA/CD25 Antibody
Product Name Alternative
IL2RA, CD25 antigen, CD25, IDDM10, IL-2 receptor subunit alpha, IL2R, Interleukin 2 receptor, alpha, IL-2-RA, IL2-RA, TCGFR, IL-2R subunit alpha, p55, TAC antigen
Reactivity
Human
Immunogen
Insect cell derived recombinant Human sIL-2Ralpha.
Target
IL2RA / CD25
Clonality
Monoclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G purified
Dilution
ELISA (4 - 8 μg/ml), IHC, IHC-Fr (5 μg/ml), IHC-P (10 μg/ml), WB (0.2 - 0.4 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Resuspension: Centrifuge vial prior to opening. Reconstitiute in sterile water to 1.0 mg/ml.. Further information: This in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure) . A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 4-8 ug/ml of this antibody will detect at least 100 pg/ml of recombinant Human sIL-2Ralpha when used in conjunction with compatible secondary reagents. Western Blot: To detect Human sIL-2Ralpha by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human sIL-2Ralpha is 2-4 ng/lane, under non-reducing conditions.
Tested Applications
ELISA, IHC, IHC-Fr, IHC-P, WB
Host or Source
Mouse
Preservative
Lyophilized from Sterile Solution PBS
Isotype
IgG2a, k

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