Bromophenol blue

Bromophenol blue

• CAS Number: 115-39-9
• UNSPSC Description: Bromophenol blue is an acid phthalein dye, and it is used as a tracking dye for electrophoresis. Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6. Bromophenol blue is used as a sensor for the determination of several compounds including ammonia, drugs, proteins and amino acids. The binding of Bromophenol blue to proteins is accompanied by the appearance of a strong absorbance at 610 nm[1][2].
• Target Antigen: Fluorescent Dye
• Type: Dye Reagents
• Related Pathways: Others
• Field of Research: Others
• Assay Protocol: https://www.medchemexpress.com/bromophenol-blue.html
• Solubility: DMSO : 50 mg/mL (ultrasonic)
• Smiles: O=S1(OC(C2=CC(Br)=C(O)C(Br)=C2)(C3=CC(Br)=C(O)C(Br)=C3)C4=CC=CC=C41)=O
• Molecular Weight: 669.96
• References & Citations: [1]Dangui AZ, et, al. Preferential solvation bromophenol blue in water-alcohol binary mixture. Spectrochim Acta A Mol Biomol Spectrosc. 2018 Oct 5; 203: 333-341.|[2]Flores R. A rapid and reproducible assay for quantitative estimation of proteins using bromophenol blue. Anal Biochem. 1978 Aug 1;88(2):605-11.
• Shipping Conditions: Room Temperature
• Storage Conditions: RT, stored under nitrogen
• Clinical Information: No Development Reported