Bromophenol blue

• Description:

  Bromophenol blue is an acid phthalein dye, and it is used as a tracking dye for electrophoresis. Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6. Bromophenol blue is used as a sensor for the determination of several compounds including ammonia, drugs, proteins and amino acids. The binding of Bromophenol blue to proteins is accompanied by the appearance of a strong absorbance at 610 nm[1][2].

• UNSPSC:

  12171500

• Hazard Statement:

  H302, H315, H319, H335

• Target:

  Fluorescent Dye

• Type:

  Dye Reagents

• Related Pathways:

  Others

• Field of Research:

  Others

• Assay Protocol:

  https://www.medchemexpress.com/bromophenol-blue.html

• Concentration:

  10mM

• Solubility:

  DMSO : 50 mg/mL (ultrasonic)

• Smiles:

  O=S1 (OC (C2=CC (Br) =C (O) C (Br) =C2) (C3=CC (Br) =C (O) C (Br) =C3) C4=CC=CC=C41) =O

• Molecular Formula:

  C19H10Br4O5S

• Molecular Weight:

  669.96

• Precautions:

  H302, H315, H319, H335

• References & Citations:

  [1]Dangui AZ, et, al. Preferential solvation bromophenol blue in water-alcohol binary mixture. Spectrochim Acta A Mol Biomol Spectrosc. 2018 Oct 5; 203: 333-341.|[2]Flores R. A rapid and reproducible assay for quantitative estimation of proteins using bromophenol blue. Anal Biochem. 1978 Aug 1;88 (2) :605-11.

• Shipping Conditions:

  Room Temperature

• Storage Conditions:

  RT, stored under nitrogen

• Scientific Category:

  Dye Reagents

• Clinical Information:

  No Development Reported

• CAS Number:

  115-39-9