Bromophenol blue

• Description :

  Bromophenol blue is an acid phthalein dye, and it is used as a tracking dye for electrophoresis. Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6. Bromophenol blue is used as a sensor for the determination of several compounds including ammonia, drugs, proteins and amino acids. The binding of Bromophenol blue to proteins is accompanied by the appearance of a strong absorbance at 610 nm[1][2].

• UNSPSC :

  12171500

• Hazard Statement :

  H302, H315, H319, H335

• Target :

  Fluorescent Dye

• Type :

  Dye Reagents

• Related Pathways :

  Others

• Field of Research :

  Others

• Assay Protocol :

  https://www.medchemexpress.com/bromophenol-blue.html

• Concentration :

  10mM

• Solubility :

  DMSO : 50 mg/mL (ultrasonic)

• Smiles :

  O=S1(OC(C2=CC(Br)=C(O)C(Br)=C2)(C3=CC(Br)=C(O)C(Br)=C3)C4=CC=CC=C41)=O

• Molecular Formula :

  C19H10Br4O5S

• Molecular Weight :

  669.96

• Precautions :

  H302, H315, H319, H335

• References & Citations :

  [1]Dangui AZ, et, al. Preferential solvation bromophenol blue in water-alcohol binary mixture. Spectrochim Acta A Mol Biomol Spectrosc. 2018 Oct 5; 203: 333-341.|[2]Flores R. A rapid and reproducible assay for quantitative estimation of proteins using bromophenol blue. Anal Biochem. 1978 Aug 1;88 (2) :605-11.

• Shipping Conditions :

  Room Temperature

• Storage Conditions :

  RT, stored under nitrogen

• Scientific Category :

  Dye Reagents

• Clinical Information :

  No Development Reported

• CAS Number :

  [115-39-9]