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R-Phycoerythrin Antibody

CAT: 0013-GTR18207346Size: 1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18207346Size:1 mg
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Description
R-Phycoerythrin antibody
Product Name Alternative
Goat anti R-Phycoerythrin Antibody, goat anti-R-Phycoerythrin Antibody, goat anti Phycoerythrin
UniProt
Q7SIG0
Immunogen
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/mL
Dilution
ELISA: 1:20,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using a R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and R-Phycoerythrin conjugated IgG. This antibody will cross react with B-Phycoerythrin. Reactivity with other phycobiliproteins is unknown.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-R-Phycoerythrin Antibody is tested by ELISA and suitable for western blotting, fluorescent assays, immunoprecipitation, immunodiffusion, conjugation to magnetic particles (beads) and most other immunological methods requiring lot-to-lot consistency, high titer and specificity. Although not specifically tested, this reagent should also be useful for FACS and other fluorescent immunoassays. R-Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents. Reaction with low molecular fragments is typically noted by western blot.
Tested Applications
ELISA, IHC, WB
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · Q7SIG0

R-phycoerythrin alpha chain

PHEA_AGACH · Agarophyton chilense

View on UniProt ↗
Primary accession
Q7SIG0
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
rpeA
Protein existence
1: Evidence at protein level
Organism
Agarophyton chilense (Red seaweed)
Taxonomy ID
2510777
Alternative names
—
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

Technical term

3D-structure

Cellular component

Antenna complexChloroplastMembranePhycobilisomePlastidThylakoid

Ligand

Bile pigmentChromophore

Biological process

Electron transportPhotosynthesisTransport

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