Mouse anti Human CD8 FITC - CD45RA PE

Mouse anti Human CD8 FITC - CD45RA PE

• Background: CD8 is useful for the Identification of Human T cells suppressor/cytotoxic expressing the 32 kDa M.W. surface antigen. While CD45RA recognizes MW. 220-kdalton of the leucocyte antigen (LCA). CD45RA antigen is a member of the CD45 antigen family that also includes CD45, CD45RO, and CD45RB antigens. CD45RA is expressed on approximately 75% of CD8 lymphocytes and 50% of CD4 lymphocytes, all B cells and NK Cells. CD45RA antigen is expressed on naive T lymphocytes, antigen density decreases upon in vitro activation. A decrease antigen density has been demonstrated for CD4+CD45RA+subset with active multiple sclerosis._x000B_
• Synonyms: CD8 FITC - CD45RA PE
• CAS Number: 9007-83-4
• UniProt: P01732, P08575
• Host: Mouse
• Species Reactivity: Human
• Isotype: IgG1
• Clone: 17D8/BHKG-2
• Conjugation: Bi-Test (FITC/RPE) Reagent
• Type: Bi-Testª Reagents (FITC/RPE)
• Applications: Flow Cytometry
• Field of Research: Flow Cytometry
• Purification Method: Protein A/G Chromatography
• Assay Principle: Add 10 µL of MAB / 100 µL of 106 PBMC in PBS. Mix Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. Add 10 µLof MAB / 100 µL of Whole Blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse Whole Blood and analyze or fix with 0.5% v/v paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
• Stability: See expiration date on vial
• Concentration: Titered for flow cytometry
• Form: Provided as solution in phosphate buffered saline with 0,08% sodium azide and 0,2% carrier protein
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• References & Citations: 1. Knowles RW. Immunochemical analysis of the T cell-specific antigens. In : Reinhert EL, Haynes BF, Nadl LM and Bernstein ID. eds. Leukocyte Typing II, Human T Lymphocytes. New York, NY: Springer-Verlag; 1986:259. 2. Kurrle R. Cluster Report:CD3. In:Knapp W, Dorken B, Gilks WR, Reiber EP, Schmidt RE, Stein H, and von dem Borne AEG Kr, eds. Leukocyte Typing IV, White cell Differentiation Antigens. Oxford, England: Oxford Press 1989:293. 3. T cell receptor/CD3-signaling induces death by apoptosis in Human T cell receptor gamma delta + Tcells. Janssen O, Wesselborg S, Heckl-Ostreicher B, Pechhold K, Bender A, Schondelmaier S, Moldenhauer G, Kabelitz D I Immunol. 1991 Jan146(1):35-9. 4. Clonal analysis of Human CD4-CD8-CD3- thymocytes highly purified from postnatal thymus Hori T, Spits H J. Immunol. 1991 Apr 146(7):2116-21. 5. Molecular cloning of the CD3 zeta subunit identifies a CD3 zeta-related product in thymus-derived cells Jin YJ, Claton LK, Howard FD, Koyasu S, Sieh M, Steinbrich R, Tarr GE, Reinherz EL. Proc Natl Acad Sci USA 1990 Ma: 87(9):3319-23. 6. Leucocyte Typing IV: White Cell Differentiation Antigens, Schwinzer R. Cluster report: CD45/CD45R, Knapp W., Dorken B., Gilks W.R.,et al, eds. Oxford University Press; 1989:628-634 7. CD45 isoform expression on Human neonatal T cells: expression and turnover of CD45 isoforms on neonatal versus adult T cells after activation. Cell Immunol 1992 Jun;142(1):114-24 Yamada A; Kaneyuki T; Hara A; Rothstein DM; Yokoyama MM 8. Transient accumulation and subsequent rapid loss of messenger RNA encoding high molecular mass CD45 isoforms after T cell activation. J Immunol 1992 Mar 15;148(6):1898-905 Deans JP; Serra HM; Shaw J; Shen YJ; Torres RM; Pilarski LM 9. Patterns of membrane CD45 isoform expression by leukaemic blasts and normal mature myeloid cells. Int J Hematol 1992 Jun;55(3):235-42 Master PS; Richards SJ; Roberts BE; Scott CS 10. CD45 isoform expression during T cell development in the thymus Eur J Immunol 1992 Jul;22(7):1843-50 Fujii Y; Okumura M; Inada K; Nakahara K; Matsuda H 11. Functional subsets of T cells defined by isoforms of CD45 Biochem Soc Trans 1992 Feb;20(1):184-7 Beverley PC; Daser A; Michie CA; Wallace D
• Shipping Conditions: Ambient Temperature, do not freeze, refrigerate upon arrival
• Storage Conditions: Product should be stored at 4-8ºC; DO NOT FREEZE
• Functional Analysis: Flow Cytometry Staining