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Human Lung Tumor Blot V

CAT: 0223-1514Size: 1 EachDry Ice: NoHazardous: No
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CAT#:0223-1514Size:1 Each
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Background
The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries the cell line A549 (human lung carcinoma), and 3 different human lung tumor tissue lysates and matched normal lysates (from non-tumor lung tissue from near the tumor) with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The lung tumor tissues included in the blot are 1 small cell carcinoma, 1 differentiated carcinoma, and 1 pleomorphic carcinoma.
Type
Immunoblots
Shipping Conditions
Room Temp
Storage Conditions
Store at 4°C. The blot can be reused after stripping.
Applications Notes
For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
Tested Applications
WB
Species
Human

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