Lung Tissue Lysate (Normal)

• Background:

  Lung tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μ g/ml of aprotinin, 5 μ g/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β -mercaptoethanol.

• Type:

  Lysates

• Concentration:

  2 mg/mL

• Buffer:

  Lung Tissue Lysate (Normal) is supplied in SDS sample buffer containing 5% β -mercaptoethanol.

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 2-8˚ C for continuous use. For extended storage, freeze working aliquots at -70˚ C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Liquid