Tryptophan synthase Recombinant Protein

• Background:

  Tryptophan synthase is a multienzyme alpha2 beta 2 complex composed of two protein subunit. Tryptophan synthase catalyzes the last two steps in the synthesis of L-tryptophan (L-Trp) . The alpha-subunit catalyzes cleavage of 3-indole-d-glycerol 3′-phosphate (IGP) to give indole and D-glyceraldehyde 3′-phosphate (G3P) . Indole is then transferred through a 25-Å hydrophobic tunnel to the beta -subunit. The beta 2 subunit contains pyridoxal 5'-phosphate and catalyzes several pyridoxal 5'-phosphate-dependent reactions, including/3-elimination reactions 6 and a thiol-dependent transamination reaction. This enzyme is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from Animalia. As humans do not have tryptophan synthase, this enzyme has been explored as a potential drug target.

• NCBI Gene ID:

  946204

• Swiss Prot:

  P0A877, P0A879

• Accession Number:

  P0A877, P0A879

• Tag:

  N-6 His tag

• Type:

  Recombinant Proteins

• Source:

  E. coli

• Buffer:

  Lyophilized from a 0.2 um filtered solution of PBS, pH7.4. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O.

• Shipping Conditions:

  Room Temp

• Calculated Molecular Weight:

  72.5 kD

• Applications Notes:

  This recombinant protein can be used for biological assays. For research use only.

• NCBI Official Name:

  Tryptophan synthase, alpha subunit

• NCBI Organism:

  Escherichia coli str. K-12 substr. MG1655

• Other Product Names:

  Tryptophan synthetase, Tryptophan synthase

• Species:

  E. coli

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3