Biotinylated TREM2 Recombinant Protein

• Background:

  Triggering receptor expressed on myeloid cells 2 (TREM2) is a cell surface receptor of the immunoglobulin superfamily. The TREM2 is found in various tissue macrophages, such as CNS microglia, bone osteoclasts, alveolar, peritoneal and intestinal macrophages. TREM2 is also present on cultured bone marrow-derived macrophages and monocyte-derived dendritic cells. Some research have identified a rare variant of TREM2 that is a risk factor for Alzheimer disease (AD), which is the most common form of late-onset dementia.The extracellular region of TREM2 contains a single immunoglobulin superfamily domain and binds polyanionic ligands, such as bacterial lipopolysaccharide (LPS) and phospholipids8. Upon ligand binding, TREM2 transmits intracellular signals through an adaptor, DAP12 (also known as TYRO protein tyrosine kinase-binding protein (TYROBP) ), which is associated with the transmembrane region of TREM2 and which recruits the protein tyrosine kinase SYK through its cytosolic immunoreceptor tyrosine-based activation motifs (ITAMs) . TREM2 is a pro-tumorigenic marker of tumor-infiltrating macrophages in mouse models and human tumors that can be targeted to curb tumor growth and improve the efficacy of checkpoint blockade therapy while remodeling the landscape of tumor-infiltrating macrophages.

• Tag:

  His Tag & Avi Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Apoptosis, Cancer, Homeostasis, Immunology, Other

• Purity:

  >90% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  21.0 kDa

• Applications Notes:

  This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 21.0 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.

• Background Reference 01:

  Martina Molgora, Ekaterina Esaulova et al. TREM2 Modulation Remodels the Tumor Myeloid Landscape Enhancing Anti-PD-1 Immunotherapy.[J]. CELL, 2020.

• Background Reference 02:

  Colonna M , Wang Y . TREM2 variants: new keys to decipher Alzheimer disease pathogenesis.[J]. Nature reviews Neuroence, 2016.

• Background Reference 03:

  Karanfilian L , Tosto M G , Malki K . The Role of TREM2 in Alzheimer's Disease; Evidence from Transgenic Mouse Models[J]. Neurobiology of Aging, 2019.

• Background Reference 04:

  Guerreiro R , Wojtas A , Bras J , et al. TREM2 variants in Alzheimer's disease.[J]. New England Journal of Medicine, 2013, 368 (2) .

• Other Product Names:

  Triggering receptor expressed on myeloid cells 2/TREM2/TREM-2

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3