CD95 Recombinant Protein

• Background:

  The Fas is also known as FAS receptor (FasR), apoptosis antigen 1 (APO-1 or APT), cluster of differentiation 95 (CD95) or tumor necrosis factor receptor superfamily member 6 (TNFRSF6) . is a death receptor on the surface of cells that leads to programmed cell death (apoptosis) . It is one of two apoptosis pathways, the other being the mitochondrial pathway. FasR is located on chromosome 10 in humans and 19 in mice. Similar sequences related by evolution (orthologs) are found in most mammals. Fas forms the death-inducing signaling complex (DISC) upon ligand binding. Membrane-anchored Fas ligand trimer on the surface of an adjacent cell causes trimerization of Fas receptor. This event is also mimicked by binding of an agonistic Fas antibody, though some evidence suggests that the apoptotic signal induced by the antibody is unreliable in the study of Fas signaling. To this end, several clever ways of trimerizing the antibody for in vitro research have been employed.Upon ensuing death domain (DD) aggregation, the receptor complex is internalized via the cellular endosomal machinery. This allows the adaptor molecule FADD to bind the death domain of Fas through its own death domain. Recently, Fas has also been shown to promote tumor growth, since during tumor progression, it is frequently downregulated or cells are rendered apoptosis resistant. Cancer cells in general, regardless of their Fas apoptosis sensitivity, depend on constitutive activity of Fas. This is stimulated by cancer-produced Fas ligand for optimal growth.

• NCBI Gene ID:

  355

• Swiss Prot:

  P25445

• Accession Number:

  AAH12479.1

• Tag:

  C-Fc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Apoptosis, Cancer, Cell Cycle, Immunology, Innate Immunity, Neuroscience, Signal Transduction, Other

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  50 mM tris, 100 mM glycine, pH7.5

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  42.8 kDa

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  Fas cell surface death receptor

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Wajant H, 2002, Science 296 (5573) : 1635–6.

• Background Reference 02:

  Huang B, et al., 1996, Nature 384 (6610) : 638–41.

• Background Reference 03:

  Chen L, et al., 2010, Nature 465 (7297) : 492–6.

• Other Product Names:

  FAS, ALPS1A, APO1, APT1, CD95, FAS1, FASTM, TNFRSF6, FasR

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3