Biotinylated PSGL-1 / CD162 Recombinant Protein

• Background:

  P-selectin glycoprotein ligand 1 (PSGL-1) is also known as Selectin P ligand (SELPLG), CD antigen CD162. PSGL-1 is disulfide-linked homodimer which is the high affinitycounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 / SELPLG interacts with P-, E- and L-selectins, through their lectin/EGF domains, is required for promoting recruitment and rolling of leukocytes. These interactions require sialyl Lewis X glycan modification but there is a differing dependence for tyrosine sulfations. Dimerization appears not to be required for P-selectin/SELP binding. PSGL-1 can interact with SNX20, MSN and SYK. PSGL-1 / SELPLG mediate the activation of SYK by SELPLG.

• NCBI Gene ID:

  6404

• Swiss Prot:

  Q14242

• Accession Number:

  NP_002997.2

• Tag:

  His Tag & Avi Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >90% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  32.4 kDa

• Applications Notes:

  This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 32.4 kDa. The protein migrates as 55-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  SelectinPligand

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  PSGL1, CD162, SELPLG, Selectin P ligand

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3