TM Yeast Pst1 Recombinant Protein

• Background:

  Protoplast secreted protein 1 (Pst1) is an anchored plasma membrane protein. Pst1 was previously identified as a protein secreted by yeast regenerating protoplasts, which suggests a role in cell wall construction (1) . Pst1 is a protein with 444 amino acids and is attached to yeast cell wall via a glycosylphosphatidylinisotol (GPI) anchor (2) . Pst1 contains 15 potential N-linked glycosylation sites and is heavily glycosylated. It migrates at around 200 kDa on SDS-PAGE when produced in wild type S. cerevisiae. Our Pst1 protein was expressed in a genetically manipulated triple-mutant (TM) S. cerevisiae stain (Δoch1 Δmnn1 Δmnn4), which results in the production of sole Man8GlcNAc2 carbohydrate structures and Pst1 migration at approximately 100 kDa. When produced in the TM yeast, Pst1 can be recognized by several HIV-1 broadly neutralizing antibodies, including 2G12 and recently identified glycan-specific PGT antibodies (3) . Among several heavily N-glycosylated TM yeast glycoproteins, Pst1 shows high affinity for 2G12 and efficiently inhibits gp120 interactions with 2G12 and DC-SIGN, and it also blocks 2G12-mediated neutralization of HIV-1 pseudoviruses (4) .

• NCBI Gene ID:

  851625

• Swiss Prot:

  Q12355

• Accession Number:

  Q12355.1

• Type:

  Recombinant Proteins

• Source:

  S. cerevisiae TM strain (Δ och1 Δ mnn1 Δ mnn4)

• Field of Research:

  Infectious Disease

• Purity:

  ~95%

• Buffer:

  Lot specific, TBS or PBS

• Shipping Conditions:

  Dry Ice

• Storage Conditions:

  Store in working aliquots at -70˚ C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

• Calculated Molecular Weight:

  100 kDa on SDS-PAGE

• Applications Notes:

  This recombinant protein can be used for WB and ELISA. For research use only.

• NCBI Official Name:

  Protoplast secreted protein 1

• NCBI Organism:

  Saccharomyces cerevisiae S288c

• Background Reference 01:

  Pardo, M, Monteoliva L, Pla J, et al., Two-dimensional analysis of proteins secreted by Saccharomyces cerevisiae regenerating protoplasts: a novel approach to study the cell wall. Yeast. 1999 Apr;15 (6) :459-72.

• Background Reference 02:

  Pardo, M, Monteoliva L, Vázquez P, et al., PST1 and ECM33 encode two yeast cell surface GPI proteins important for cell wall integrity. Microbiology. 2004 Dec;150 (12) :4157-70.

• Background Reference 03:

  Luallen, RJ, Lin JQ, Fu H, et al., An Engineered Saccharomyces cerevisiae Strain Binds the Broadly Neutralizing Human Immunodeficiency Virus Type 1 Antibody 2G12 and Elicits Mannose-Specific gp120-Binding Antibodies. J Virol. 2008 July; 82 (13) : 6447-6457.

• Background Reference 04:

  Luallen RJ, Fu H, Agrawal-Gamse C, et al., A Yeast Glycoprotein Shows High-Affinity Binding to the Broadly Neutralizing Human Immunodeficiency Virus Antibody 2G12 and Inhibits gp120 Interactions with 2G12 and DC-SIGN. J Virol. 2009 May; 83 (10) : 4861-4870.

• Other Product Names:

  TM Yeast Pst1 Antibody: HPF2, HPF2, YDR055W, D4214, YD9609.09, Cell wall mannoprotein PST1, Haze protective factor 2

• Tested Applications:

  ELISA, WB

• Species:

  Yeast

• Protein ID:

  17380259

• Physical Properties:

  Liquid

• CAS Number:

  9000-83-3