PD-1 / PDCD1 Recombinant Protein

• Background:

  Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.

• NCBI Gene ID:

  5133

• Swiss Prot:

  Q15116

• Accession Number:

  NP_005009.2

• Tag:

  Mouse IgG Fc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Apoptosis, Cancer, Cell Cycle, Immunology

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  42.5 kDa

• Applications Notes:

  This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 42.5 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  Programmed cell death 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ishida Y., et al., 1992, EMBO J. 11 (11) : 3887–95.

• Background Reference 02:

  Blank C., et al., 2007, Cancer Immunol. Immunother. 56 (5) : 739–45.

• Background Reference 03:

  Agata Y., et al., 1996, Int. Immunol. 8 (5) : 765–72.

• Background Reference 04:

  Freeman GJ., et al., 2000, J. Exp. Med. 192 (7) : 1027–34.

• Other Product Names:

  PDCD1, PD1, CD279, SLEB2

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3