Alpha-Synuclein Recombinant Protein

• Background:

  Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca2+ release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity.

• NCBI Gene ID:

  6622

• Swiss Prot:

  P37840

• Accession Number:

  NP_000336.1

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  E. coli

• Purity:

  >90% as determined by SDS-PAGE.

• Buffer:

  100 mM NaAC, pH7.0

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  16.3 kDa

• Applications Notes:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.3 kDa. The protein migrates as 19 kDa under reducing (R) condition (SDS-PAGE) .

• NCBI Official Name:

  Synucleinalpha

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Butler B, et al. 2015. J Biol Chem. 290 (49) :29542-54.

• Background Reference 02:

  Logan T, 2017. Nat Neurosci. 20 (5) :681-689.

• Background Reference 03:

  Huang CC, et al. 2018. J Cell Sci. 131 (23) .

• Background Reference 04:

  Burré J, et al. 2010. Science. 329 (5999) :1663-7.

• Other Product Names:

  SNCA, NACP, PARK1, alpha-Synuclein

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3