ICOS / CD278 (C137S, C138S) Recombinant Protein

• Background:

  Inducible T-cell costimulator (ICOS) is also known as Activation-inducible lymphocyte immunomediatory molecule (AILIM), CD278, which belongs to the CD28 family of immune costimulatory receptors consisting of CD28, CTLA-4 and PD-1. ICOS enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. CD278 / ICOS prevents the apoptosis of pre-activated T-cells and also plays a critical role in CD40-mediated class switching of immunoglobin isotypes.

• NCBI Gene ID:

  54167

• Swiss Prot:

  Q9WVS0

• Accession Number:

  NP_059508.2 (C137S, C138S)

• Tag:

  Fc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  Tris with Glycine, Arginine and NaCl, pH7.5

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  40.6 kDa

• Applications Notes:

  This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 40.6 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  Inducible T cell co-stimulator

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Hutloff A., et al., 1999, Nature 397:263-266.

• Background Reference 02:

  Tezuka K., et al., 2000, Biochem. Biophys. Res. Commun. 276:335-345.

• Background Reference 03:

  Beier K.C., et al., 2000, Eur. J. Immunol. 30:3707-3717.

• Other Product Names:

  ICOS, CD278, AILIM, Inducible T-cell costimulator

• Tested Applications:

  ELISA, WB

• Species:

  Mouse

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3