Erythropoietin Recombinant Protein

• Background:

  Human Erythropoietin (EPO) is also known as EP, erythropoetin or erthropoyetin, and is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. EPO is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. is synthesized by renal peritubular cells in adults, with a small amount being produced in the liver. Regulation is believed to rely on a feed-back mechanism measuring blood oxygenation. Constitutively synthesized transcription factors for EPO, known as hypoxia-inducible factors (HIFs), are hydroxylated and proteosomally digested in the presence of oxygen. It binds to the erythropoietin receptor (EpoR) on the red cell surface and activates a JAK2 cascade. Erythropoietin has its primary effect on red blood cells by promoting red blood cell survival through protecting these cells from apoptosis. It also cooperates with various growth factors involved in the development of precursor red cells. EPO has a range of actions including vasoconstriction-dependent hypertension, stimulating angiogenesis, and inducing proliferation of smooth muscle fibers. It has also been shown that erythropoietin can increase iron absorption by suppressing the hormone hepcidin. Erythropoietin has been shown to interact with the Erythropoietin receptor as its mechanism of action within the body. erythropoietin plays an important role in the brain's response to neuronal injury. EPO is also involved in the wound healing process.

• NCBI Gene ID:

  2056

• Swiss Prot:

  P01588

• Accession Number:

  AAH93628.1

• Tag:

  Tag free

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Cancer, Cell Cycle, Obesity, Signal Transduction

• Purity:

  >97% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  18.4 kDa

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  Erythropoietin

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Jacobson LO, et al., 1957, Nature 179 (4560) : 633–4.

• Background Reference 02:

  Fisher JW, et al., 1996, British journal of haematology 95 (1) : 27–32.

• Background Reference 03:

  Jelkmann W ., 2007, Eur. J. Haematol. 78 (3) : 183–205.

• Background Reference 04:

  Ashby DR, et al., 2010, Haematologica 95 (3) : 505–8.

• Other Product Names:

  EPO, EP, MVCD2, Erythropoietin, Erythropoetin, Erthropoyetin, Hematopoietin, Hemopoietin

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3