Flt-3 / Flk-2 Recombinant Protein

• Background:

  Flt-3 (Receptor-type tyrosine-protein kinase FLT3) is also known as FLK-2 (Fetal liver kinase-2), STK-1 (Stem cell tyrosine kinase 1), CD135. FLT3 is a cytokine receptor which belongs to the receptor tyrosine kinase class III. Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.

• NCBI Gene ID:

  2322

• Swiss Prot:

  P36888

• Accession Number:

  NP_004110.2

• Tag:

  Fc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  Tris with Glycine, Arginine and NaCl, pH7.5

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  84.8 kDa

• Applications Notes:

  This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 84.8 kDa. The protein migrates as 110-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  Fms related receptor tyrosine kinase 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Zheng R, et al. 2011. Oncogene. 30 (38) :4004-14.

• Background Reference 02:

  Mizuki M, et al. 2000. Blood. 96 (12) :3907-14.

• Background Reference 03:

  Small D, et al. 1994. Proc Natl Acad Sci U S A. 91 (2) :459-63.

• Other Product Names:

  Flt-3, Flk-2, STK-1, CD135, FLK2, FLT-3

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3