CD59 Recombinant Protein

• Background:

  CD59, also known as membrane attack complex inhibition factor (MACIF), Protectin, 1F5 antigen, HRF-20 and MIRL. CD59 has been identified as a glycosylphosphatidylinositol-anchored membrane protein that acts as an inhibitor of the formation of the membrane attack complex to regulate complement activation. Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Immunology, Signal Transduction

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  10.8 kDa

• Applications Notes:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 10.8 kDa. The protein migrates as 15-18 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Background Reference 01:

  Zhang R, et al. Future Oncol. 2018. 14 (8) :781-791.

• Background Reference 02:

  Höchsmann B, et al. Hematol Oncol Clin North Am. 2015. 29 (3) :495-507.

• Background Reference 03:

  Fülöp G, et al. Biomolecules. 2018. 8 (2) .

• Other Product Names:

  CD59,16.3A5,1F5, EJ16, EJ30, EL32, FLJ38134, FLJ92039, G344, HRF20, MAC-IP, MACIF, MEM43, MGC2354, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18-20, Protectin

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3