CD55 Recombinant Protein

• Background :

  CD55 is also known as DAF or decay­accelerating factor, is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. CD55 /DAF is a 70 kDa membrane protein that regulates the complement system on the cell surface. It prevents the assembly of the C3bBb complex (the C3-convertase of the alternative pathway) or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex. This glycoprotein is broadly distributed among hematopoietic and non-hematopoietic cells. It is a determinant for the Cromer blood group system. CD55 is known to bind CD97 via the first SCR. It also binds physiologically­generated C3 convertases with its second and third SCRs. Binding results in an accelerated “decay”, or dissociation of active C3 convertases, thus blocking the development of C’ attack complexes on nonforeign cells. Finally, viruses and bacteria are also known to utilize multiple SCR sites for infection.

• NCBI Gene ID :

  1604

• Swiss Prot :

  P08174

• Accession Number :

  NP_000565.1

• Tag :

  His Tag

• Type :

  Recombinant Proteins

• Source :

  HEK293 cells

• Field of Research :

  Immunology, Signal Transduction

• Purity :

  >95% as determined by SDS-PAGE.

• Buffer :

  PBS, pH7.4

• Shipping Conditions :

  Room Temp

• Storage Conditions :

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight :

  35.8 kDa

• Applications Notes :

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name :

  CD55 molecule, decay accelerating factor for complement (Cromer blood group)

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Medof ME, et al., 1987, Proc. Natl. Acad. Sci. U.S.A. 84 (7) : 2007–11.

• Background Reference 02 :

  Karnauchow TM, et al., 1996, J. Virol. 70 (8) : 5143–52.

• Background Reference 03 :

  Spiller OB, et al., 2000, J. Infect. Dis. 181 (1) : 340–3.

• Background Reference 04 :

  Williams, P. et al., 2003, J. Biol. Chem. 278:10691.

• Other Product Names :

  CD55, DAF, CR, CROM, TC

• Tested Applications :

  WB

• Species :

  Human

• Physical Properties :

  Lyophilized

• CAS Number :

  9000-83-3