CD39 / ENTPD1 Recombinant Protein

• Background:

  CD39 is also known as Ectonucleoside triphosphate diphosphohydrolase 1, ENTPD1, NTPDase 1, Ecto-ATPDase 1, in the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well. NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker, but it is also present on the surface of natural killer cells, T cells, and some endothelial cells.Regulatory T cells (Tregs) mediate immunosuppression through multiple, non-redundant, cell-contact dependent and independent mechanisms, a growing body of evidence suggests an important role for the CD39-CD73-adenosine pathway. CD39 ectonucleotidase is the rate-limiting enzyme of a cascade leading to the generation of suppressive adenosine that alters CD4 and CD8 T cell and natural killer cell antitumor activities.

• NCBI Gene ID:

  953

• Swiss Prot:

  P49961

• Accession Number:

  NP_001767.3

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Immunology, Signal Transduction

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  Tris and NaCl, pH8.0

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  52.3 kDa

• Applications Notes:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 52.3 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  Ectonucleoside triphosphate diphosphohydrolase 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Kaczmarek E, et al. 1996, J Biol Chem, 271 (51) :33116-22.

• Background Reference 02:

  Lima NF, et al. 2018, PLoS Negl Trop Dis., 12 (3) :e0006327.

• Background Reference 03:

  Bastid J, et al. 2013, Oncogene, 32 (14) :1743-51.

• Other Product Names:

  CD39, ENTPD1, NTPDase 1, Entpd1, Ecto-ATPDase 1, Ecto-ATPase 1

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3