CD36 Recombinant Protein

• Background:

  CD36 (Cluster of Differentiation 36) is also known as platelet membrane glycoprotein IV (GPIV), fatty acid translocase (FAT), thrombospondin receptor, collagen receptor, and scavenger receptor class B, member 3 (SRB3), is a member of the class B scavenger receptor family of cell surface proteins. The human CD36 gene encodes a single chain 472 amino acid residue protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.CD36 is found on platelets, erythrocytes, monocytes, differentiated adipocytes, mammary epithelial cells, spleen cells and some skin microdermal endothelial cells. CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells, beta-amyloid fibrils (fAβ), collagens I and IV, and Plasmodium falciparum infected erythrocytes. CD36 is required for the anti-angiogenic effects of thrombospondin1 In the corneal neovascularization assay. On binding a ligand the protein and ligand are internalized. This internalization is independent of macropinocytosis and occurs by an actin dependent mechanism requiring the activation Src-family kinases, JNK and Rho-family GTPases. CD36 ligands have also been shown to promote sterile inflammation through assembly of a Toll-like receptor 4 and 6 heterodimer.

• NCBI Gene ID:

  948

• Swiss Prot:

  P16671

• Accession Number:

  NP_001001547.1

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Cancer, Immunology, Obesity, Signal Transduction, Inflammasomes

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  47.5 kDa

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  CD36 molecule (thrombospondin receptor)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Husemann, J. et al., 2002, Glia 40:195.

• Background Reference 02:

  Armstrong, L and P. Bornstein, 2003, Matrix. Biol. 22:63.

• Background Reference 03:

  Collins RF, et al., 2009, J. Biol. Chem. 284 (44) : 30288–97.

• Background Reference 04:

  Stewart CR, et al., 2010, Nat. Immunol. 11 (2) : 155–61.

• Other Product Names:

  CD36, SCARB3, BDPLT10, CHDS7, FAT, GP3B, GP4, GPIV, PASIV, Platelet Glycoprotein 4, glycoprotein IV, gpIV, glycoprotein IIIb, gpIIIb

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3