Products for Research Use Only

EPCAM Antibody (Biotin)

CAT: 0013-GTR18194693Size: 0.5 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18194693Size:0.5 mL
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Description
Mouse monoclonal antibody against EpCAM conjugated to Biotin
Product Name Alternative
Anti-Adenocarcinoma-associated Antigen antibody, anti-Cell Surface Glycoprotein Trop-1 antibody, anti-EGP2 antibody, anti-EGP314 antibody, anti-EGP40 antibody, anti-Epithelial Cell Adhesion Molecule antibody, anti-Epithelial Glycoprotein 314 antibody, anti-ESA antibody, anti-KSA antibody, anti-TACD1 antibody, anti-TROP1 antibody, anti-Tumor-associated Calcium Signal Transducer 1 (TACSTD1) antibody, anti-ECS-1 antibody, anti-Epidermal Surface Antigen 1 antibody, anti-ESA1 antibody, anti-FLOT2 antibody, anti-Flotillin-2 antibody, anti-Membrane Component antibody, anti-Chromosome 17 antibody, anti-Surface Marker-1 (M17S1) antibody, anti-REG-1 antibody, anti-Reggie-1 antibody, anti-Reggie-2 antibody
UniProt
P16422
Reactivity
Human
Immunogen
Small cell lung carcinoma cells
Target
EPCAM
Clonality
Monoclonal
Clone
VU-1D9
Conjugation
Biotin
Field of Research
Immunology & Inflammation
Purification
Protein G
Concentration
Purified Ab conjugated to Biotin
Dilution
Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood) ; Immunofluorescence (2-4ug/ml) ; , Immunohistochemistry (Formalin-fixed) (2-4ug/ml at RT) ; , (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Form
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Molecular Weight
40-43kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: HT29 cells or breast tumor
Tested Applications
FC, IF, IHC
Host or Source
Mouse
Preservative
Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
Isotype
IgG1
Entrez
4072

UniProtKB · P16422

Epithelial cell adhesion molecule

EPCAM_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P16422
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
EPCAM
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
Ep-CAM
EC number
—
Processing
Precursor
Secondary accessions
P18180, Q6FG26, Q6FG49, Q96C47, Q9UCD0
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Cellular component

Cell junctionCell membraneMembraneTight junction

Disease

Disease variantHereditary nonpolyposis colorectal cancer

PTM

Disulfide bondGlycoprotein

Domain

RepeatSignalTransmembraneTransmembrane helix

Molecular function

Tumor antigen